Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography
dc.contributor.author | Gambelli, L | |
dc.contributor.author | Meyer, BH | |
dc.contributor.author | McLaren, M | |
dc.contributor.author | Sanders, K | |
dc.contributor.author | Quax, TEF | |
dc.contributor.author | Gold, VAM | |
dc.contributor.author | Albers, SV | |
dc.contributor.author | Daum, B | |
dc.date.accessioned | 2020-03-13T16:25:54Z | |
dc.date.issued | 2019-11-25 | |
dc.description.abstract | Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell-cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer. | en_GB |
dc.description.sponsorship | ERC | en_GB |
dc.identifier.citation | Vol. 116, pp. 25278 - 25286 | en_GB |
dc.identifier.doi | 10.1073/pnas.1911262116 | |
dc.identifier.grantnumber | MICROROBOTS | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/120256 | |
dc.language.iso | en | en_GB |
dc.publisher | National Academy of Sciences | en_GB |
dc.rights | Copyright © 2019 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY). | en_GB |
dc.subject | S-layers | en_GB |
dc.subject | electron cryotomography | en_GB |
dc.subject | Sulfolobus | en_GB |
dc.subject | archaea | en_GB |
dc.subject | subtomogramaveraging | en_GB |
dc.title | Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-03-13T16:25:54Z | |
dc.identifier.issn | 0027-8424 | |
dc.description | This is the final version. Available from the National Academy of Sciences of the United States of America via the DOI in this record. | en_GB |
dc.identifier.eissn | 1091-6490 | |
dc.identifier.journal | Proceedings of the National Academy of Sciences of the United States of America | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-10-24 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-10-24 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-03-13T16:21:57Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2020-03-13T16:26:00Z | |
refterms.panel | B | en_GB |
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Except where otherwise noted, this item's licence is described as Copyright © 2019 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).