Show simple item record

dc.contributor.authorWall, BT
dc.contributor.authorCruz, AM
dc.contributor.authorOtten, B
dc.contributor.authorDunlop, MV
dc.contributor.authorFulford, J
dc.contributor.authorPorter, C
dc.contributor.authorAbdelrahman, DR
dc.contributor.authorFrancis, SB
dc.contributor.authorDirks, ML
dc.date.accessioned2020-05-05T10:35:20Z
dc.date.issued2020-04-18
dc.description.abstractCONTEXT: Anabolic resistance is mechanistically implicated in muscle disuse atrophy. OBJECTIVE: Assess whether anabolic resistance is associated with reduced postprandial amino acid uptake or exacerbated by excess lipid availability. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: Twenty men underwent 7 days of forearm immobilization while consuming a eucaloric (CON; n=11) or high-fat overfeeding (HFD; n=9; 50% excess energy as fat) diet (parallel design) within our Nutritional Physiology Research Unit. MAIN OUTCOME MEASURES: Pre- and post- immobilization we measured forearm muscle cross sectional area (aCSA), and postabsorptive and postprandial (3 h post ingestion of a liquid, protein rich, mixed meal) forearm amino acid metabolism using the arterialised venous-deep venous balance method and infusions of L-[ring-2H5]phenylalanine and L-[1-13C]leucine. RESULTS: Immobilization did not affect forearm muscle aCSA in either group, but tended to reduce postabsorptive phenylalanine (P=0.07) and leucine (P=0.05) net balances equivalently in CON and HFD. Mixed meal ingestion switched phenylalanine and leucine net balances from negative to positive (P<0.05), an effect blunted by immobilization (P<0.05) and to a greater extent in HFD than CON (P<0.05). Pre-immobilization, meal ingestion increased leucine rates of disappearance (Rd; P<0.05), with values peaking at 191% (from 87±38 to 254±60 µmol.min-1.100 mL forearm volume-1) and 183% (from 141±24 to 339±51 µmol.min-1.100 mL-1) above postabsorptive rates in CON and HFD, respectively, with meal induced increases not evident post-immobilization in either group (P>0.05). CONCLUSIONS: Disuse impairs the ability of a protein-rich meal to promote positive muscle amino acid balance, which is aggravated by dietary lipid oversupply. Moreover, disuse reduced postprandial forearm amino acid uptake; however, this is not worsened under high-fat conditions.en_GB
dc.description.sponsorshipPhysiological Societyen_GB
dc.identifier.citationPublished online 18 April 2020en_GB
dc.identifier.doi10.1210/clinem/dgaa184
dc.identifier.urihttp://hdl.handle.net/10871/120926
dc.language.isoenen_GB
dc.publisherOxford University Press (OUP) / Endocrine Societyen_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/32303743en_GB
dc.rights.embargoreasonUnder embargo until 18 April 2021 in compliance with publisher policyen_GB
dc.rights© Endocrine Society 2020. All rights reserved.en_GB
dc.subjectDisuseen_GB
dc.subjectamino acid uptakeen_GB
dc.subjectanabolic resistanceen_GB
dc.subjectarterio-venous balanceen_GB
dc.subjectatrophyen_GB
dc.subjectskeletal muscleen_GB
dc.subjectstable isotopesen_GB
dc.titleThe impact of disuse and high-fat overfeeding on forearm muscle amino acid metabolism in humansen_GB
dc.typeArticleen_GB
dc.date.available2020-05-05T10:35:20Z
exeter.place-of-publicationUnited Statesen_GB
dc.descriptionThis is the author accepted manuscript. The final version is available from Oxford University Press via the DOI in this recorden_GB
dc.identifier.eissn1945-7197
dc.identifier.journalJournal of Clinical Endocrinology and Metabolismen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2020-04-18
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2020-05-05T10:19:47Z
refterms.versionFCDAM
refterms.panelCen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record