Evolution of antibiotic resistance at low antibiotic concentrations including selection below the minimal selective concentration
dc.contributor.author | Stanton, IC | |
dc.contributor.author | Murray, AK | |
dc.contributor.author | Zhang, L | |
dc.contributor.author | Snape, J | |
dc.contributor.author | Gaze, WH | |
dc.date.accessioned | 2020-09-08T15:05:38Z | |
dc.date.issued | 2020-09-03 | |
dc.description.abstract | Determining the selective potential of antibiotics at environmental concentrations is critical for designing effective strategies to limit selection for antibiotic resistance. This study determined the minimal selective concentrations (MSCs) for macrolide and fluoroquinolone antibiotics included on the European Commission's Water Framework Directive's priority hazardous substances Watch List. The macrolides demonstrated positive selection for ermF at concentrations 1-2 orders of magnitude greater (>500 and <750 µg/L) than measured environmental concentrations (MECs). Ciprofloxacin illustrated positive selection for intI1 at concentrations similar to current MECs (>7.8 and <15.6 µg/L). This highlights the need for compound specific assessment of selective potential. In addition, a sub-MSC selective window defined by the minimal increased persistence concentration (MIPC) is described. Differential rates of negative selection (or persistence) were associated with elevated prevalence relative to the no antibiotic control below the MSC. This increased persistence leads to opportunities for further selection over time and risk of human exposure and environmental transmission. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Natural Environment Research Council (NERC) | en_GB |
dc.identifier.citation | Vol. 3, article 467 | en_GB |
dc.identifier.doi | 10.1038/s42003-020-01176-w | |
dc.identifier.grantnumber | BB/N504026/1 | en_GB |
dc.identifier.grantnumber | BB/L502509/1 | en_GB |
dc.identifier.grantnumber | NE/R01373X/1 | en_GB |
dc.identifier.grantnumber | NE/M011259/1 | en_GB |
dc.identifier.grantnumber | NE/N019717/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/122775 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Research | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/32884065 | en_GB |
dc.rights | © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en_GB |
dc.title | Evolution of antibiotic resistance at low antibiotic concentrations including selection below the minimal selective concentration | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-09-08T15:05:38Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version. Available on open access from Nature Research via the DOI in this record | en_GB |
dc.description | Data availability: The datasets associated with Figs. 1–6 are included in this published article as a Supplementary Data file. Metagenome sequence files have been deposited in the European Nucleotide Archive. Accession number: PRJEB38942. | en_GB |
dc.description | Code availability: Code used for metagenome analysis: FastQC; MultiQC; FLASH2; Metaphlan2; Hclust2 and ARGs-OAP v2. | en_GB |
dc.identifier.eissn | 2399-3642 | |
dc.identifier.journal | Communications Biology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2020-07-24 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2020-09-03 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-09-08T15:03:04Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2020-09-08T15:05:50Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/