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dc.contributor.authorVest, Alexander
dc.date.accessioned2014-02-12T12:33:06Z
dc.date.issued2013-09-27
dc.description.abstractJasmonic acid (JA) is a phytohormone with diverse roles in plant; development, growth and immune response. Jasmonic acid ZIM domain repressor (JAZ) proteins are important negative regulators of JA signalling. It has been shown that JAZs play an important role in the immune response of Arabidopsis thaliana to Pseudomonas syringae, as the pathogen seeks to exploit the JA signalling pathway to weaken A. thaliana's immune response. While JA signalling is vital for immune responses to necrotrophic pathogens such as Botrytis cinerea, it is antagonistic to salicylic acid signalling necessary for immune response to biotrophic pathogens such as P. syringae. Through the use of a yeast two hybrid system we sought to identify novel interactors of JAZ proteins to further map the JAZ interactome during pathogen infection and to show that the JAZ proteins interactomes change during pathogen induced stress. We screened cDNA libraries created from RNA transcripts harvested from A. thaliana during P. syringae infection, with a JAZ5 containing bait vector. We identified two putative, novel interactors of JAZ5; GSTF10 and HSC70-1 both of which have roles in plant defense and affect salicylic acid signalling. We also investigated the effect of JAZs on defence against necrotrophs by infecting At-jaz mutants with B. cinerea. We identified At-jaz5/7 and At-jaz7/10 as more susceptible to B. cinerea infection than wild type plants and hypothesized that this may be due to the removal of JAZ splice variants responsible for preventing runaway JA responses. Additionally we sought to further previous work on the role of γ-aminobutyric acid (GABA) in A. thaliana during pathogen infection. GABA has been shown to act as a source of nutrition for P. syringae but can repress pathogenesis genes. Using mass spectrometry we measured the amino acid content of A. thaliana amino acid transporter mutants inoculated with P. syringae as well as investigating the effect of GABA concentrations on P. syringae growth. We found that At-at5g41800/gat-1 plants inoculated with P. syringae and 10mM GABA exhibited significantly lowered P. syringae growth compared to inoculations of P. syringae alone.en_GB
dc.identifier.urihttp://hdl.handle.net/10871/14527
dc.language.isoenen_GB
dc.publisherUniversity of Exeteren_GB
dc.subjectPlant Pathologyen_GB
dc.subjectJasmonic Aciden_GB
dc.subjectJAen_GB
dc.subjectJAZen_GB
dc.subjectGABAen_GB
dc.subjectPseudomonas syringaeen_GB
dc.subjectBotrytis cinereaen_GB
dc.subjectMolecular Plant Pathologyen_GB
dc.subjectYeast two hybriden_GB
dc.titleThe Role of JAZs and GABA in Plant Immune Responseen_GB
dc.typeThesis or dissertationen_GB
dc.date.available2014-02-12T12:33:06Z
dc.contributor.advisorGrant, Murray
dc.publisher.departmentBiosciencesen_GB
dc.type.degreetitleMbyRes in Biosciencesen_GB
dc.type.qualificationlevelMasters Degreeen_GB
dc.type.qualificationnameMbyResen_GB


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