dc.contributor.author | Vest, Alexander | |
dc.date.accessioned | 2014-02-12T12:33:06Z | |
dc.date.issued | 2013-09-27 | |
dc.description.abstract | Jasmonic acid (JA) is a phytohormone with diverse roles in plant; development, growth
and immune response. Jasmonic acid ZIM domain repressor (JAZ) proteins are
important negative regulators of JA signalling. It has been shown that JAZs play an
important role in the immune response of Arabidopsis thaliana to Pseudomonas
syringae, as the pathogen seeks to exploit the JA signalling pathway to weaken A.
thaliana's immune response. While JA signalling is vital for immune responses to
necrotrophic pathogens such as Botrytis cinerea, it is antagonistic to salicylic acid
signalling necessary for immune response to biotrophic pathogens such as P. syringae.
Through the use of a yeast two hybrid system we sought to identify novel interactors of
JAZ proteins to further map the JAZ interactome during pathogen infection and to show
that the JAZ proteins interactomes change during pathogen induced stress. We screened
cDNA libraries created from RNA transcripts harvested from A. thaliana during P.
syringae infection, with a JAZ5 containing bait vector. We identified two putative, novel
interactors of JAZ5; GSTF10 and HSC70-1 both of which have roles in plant defense
and affect salicylic acid signalling. We also investigated the effect of JAZs on defence
against necrotrophs by infecting At-jaz mutants with B. cinerea. We identified At-jaz5/7
and At-jaz7/10 as more susceptible to B. cinerea infection than wild type plants and
hypothesized that this may be due to the removal of JAZ splice variants responsible for
preventing runaway JA responses. Additionally we sought to further previous work on
the role of γ-aminobutyric acid (GABA) in A. thaliana during pathogen infection.
GABA has been shown to act as a source of nutrition for P. syringae but can repress
pathogenesis genes. Using mass spectrometry we measured the amino acid content of A.
thaliana amino acid transporter mutants inoculated with P. syringae as well as
investigating the effect of GABA concentrations on P. syringae growth. We found that
At-at5g41800/gat-1 plants inoculated with P. syringae and 10mM GABA exhibited
significantly lowered P. syringae growth compared to inoculations of P. syringae alone. | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/14527 | |
dc.language.iso | en | en_GB |
dc.publisher | University of Exeter | en_GB |
dc.subject | Plant Pathology | en_GB |
dc.subject | Jasmonic Acid | en_GB |
dc.subject | JA | en_GB |
dc.subject | JAZ | en_GB |
dc.subject | GABA | en_GB |
dc.subject | Pseudomonas syringae | en_GB |
dc.subject | Botrytis cinerea | en_GB |
dc.subject | Molecular Plant Pathology | en_GB |
dc.subject | Yeast two hybrid | en_GB |
dc.title | The Role of JAZs and GABA in Plant Immune Response | en_GB |
dc.type | Thesis or dissertation | en_GB |
dc.date.available | 2014-02-12T12:33:06Z | |
dc.contributor.advisor | Grant, Murray | |
dc.publisher.department | Biosciences | en_GB |
dc.type.degreetitle | MbyRes in Biosciences | en_GB |
dc.type.qualificationlevel | Masters Degree | en_GB |
dc.type.qualificationname | MbyRes | en_GB |