Speckle correlation resolution enhancement of wide-field fluorescence imaging
Yilmaz, H; Putten, EGV; Bertolotti, J; et al.Lagendijk, A; Vos, WL; Mosk, AP
Date: 8 October 2015
Journal
Optica
Publisher
Optical Society of America
Publisher DOI
Abstract
High-resolution fluorescence imaging is essential in nanoscience and biological sciences. Due to the diffraction limit,
conventional imaging systems can only resolve structures larger than 200 nm. Here, we introduce a new fluorescence
imaging method that enhances the resolution by using a high-index scattering medium as an imaging ...
High-resolution fluorescence imaging is essential in nanoscience and biological sciences. Due to the diffraction limit,
conventional imaging systems can only resolve structures larger than 200 nm. Here, we introduce a new fluorescence
imaging method that enhances the resolution by using a high-index scattering medium as an imaging lens.
Simultaneously, we achieve a wide field of view. We develop a new image reconstruction algorithm that converges
even for complex object structures. We collect two-dimensional fluorescence images of a collection of 100 nm diameter
dye-doped nanospheres, and demonstrate a deconvolved Abbe resolution of 116 nm with a field of view of
10 μm × 10 μm. Our method is robust against optical aberrations and stage drifts, and therefore is well suited to
image nanostructures with high resolution under ambient conditions.
Physics and Astronomy
Faculty of Environment, Science and Economy
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