Speckle correlation resolution enhancement of wide-field fluorescence imaging

Abstract

High-resolution fluorescence imaging is essential in nanoscience and biological sciences. Due to the diffraction limit, conventional imaging systems can only resolve structures larger than 200 nm. Here, we introduce a new fluorescence imaging method that enhances the resolution by using a high-index scattering medium as an imaging lens. Simultaneously, we achieve a wide field of view. We develop a new image reconstruction algorithm that converges even for complex object structures. We collect two-dimensional fluorescence images of a collection of 100 nm diameter dye-doped nanospheres, and demonstrate a deconvolved Abbe resolution of 116 nm with a field of view of 10 μm × 10 μm. Our method is robust against optical aberrations and stage drifts, and therefore is well suited to image nanostructures with high resolution under ambient conditions.