Early endosome motility spatially organizes polysome distribution.
Higuchi, Y; Ashwin, P; Roger, Y; et al.Steinberg, G
Date: 3 February 2014
Article
Journal
Journal of Cell Biology
Publisher
Rockefeller University Press
Publisher DOI
Related links
Abstract
Early endosomes (EEs) mediate protein sorting, and their cytoskeleton-dependent motility supports long-distance signaling in neurons. Here, we report an unexpected role of EE motility in distributing the translation machinery in a fungal model system. We visualize ribosomal subunit proteins and show that the large subunits diffused ...
Early endosomes (EEs) mediate protein sorting, and their cytoskeleton-dependent motility supports long-distance signaling in neurons. Here, we report an unexpected role of EE motility in distributing the translation machinery in a fungal model system. We visualize ribosomal subunit proteins and show that the large subunits diffused slowly throughout the cytoplasm (Dc,60S = 0.311 µm(2)/s), whereas entire polysomes underwent long-range motility along microtubules. This movement was mediated by "hitchhiking" on kinesin-3 and dynein-driven EEs, where the polysomes appeared to translate EE-associated mRNA into proteins. Modeling indicates that this motor-driven transport is required for even cellular distribution of newly formed ribosomes. Indeed, impaired EE motility in motor mutants, or their inability to bind EEs in mutants lacking the RNA-binding protein Rrm4, reduced ribosome transport and induced ribosome aggregation near the nucleus. As a consequence, cell growth was severely restricted. Collectively, our results indicate that polysomes associate with moving EEs and that "off- and reloading" distributes the protein translation machinery.
Mathematics and Statistics
Faculty of Environment, Science and Economy
Item views 0
Full item downloads 0
Related items
Showing items related by title, author, creator and subject.
-
The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.
Lazzaretti, D; Veith, K; Kramer, K; et al. (Nature Research, 4 July 2016)Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. ... -
Crystal structure of the type IV secretion system component CagX from Helicobacter pylori.
Zhang, J; Fan, F; Zhao, Y; et al. (International Union of Crystallography, 28 February 2017)Helicobacter pylori, a Gram-negative bacterial pathogen prevalent in the human population, is the causative agent of severe gastric diseases. An H. pylori type IV secretion (T4S) system encoded by the cytotoxin-associated ... -
Prevalence and architecture of de novo mutations in developmental disorders
Deciphering Developmental Disorders Study (Springer Nature, 25 January 2017)The genomes of individuals with severe, undiagnosed developmental disorders are enriched in damaging de novo mutations (DNMs) in developmentally important genes. Here we have sequenced the exomes of 4,293 families containing ...