dc.contributor.author | Higuchi, Y | |
dc.contributor.author | Ashwin, P | |
dc.contributor.author | Roger, Y | |
dc.contributor.author | Steinberg, G | |
dc.date.accessioned | 2016-04-04T11:06:04Z | |
dc.date.issued | 2014-02-03 | |
dc.description.abstract | Early endosomes (EEs) mediate protein sorting, and their cytoskeleton-dependent motility supports long-distance signaling in neurons. Here, we report an unexpected role of EE motility in distributing the translation machinery in a fungal model system. We visualize ribosomal subunit proteins and show that the large subunits diffused slowly throughout the cytoplasm (Dc,60S = 0.311 µm(2)/s), whereas entire polysomes underwent long-range motility along microtubules. This movement was mediated by "hitchhiking" on kinesin-3 and dynein-driven EEs, where the polysomes appeared to translate EE-associated mRNA into proteins. Modeling indicates that this motor-driven transport is required for even cellular distribution of newly formed ribosomes. Indeed, impaired EE motility in motor mutants, or their inability to bind EEs in mutants lacking the RNA-binding protein Rrm4, reduced ribosome transport and induced ribosome aggregation near the nucleus. As a consequence, cell growth was severely restricted. Collectively, our results indicate that polysomes associate with moving EEs and that "off- and reloading" distributes the protein translation machinery. | en_GB |
dc.description.sponsorship | This work was supported by Wellcome Trust (097835/Z/11/Z) and the
Biotechnology and Biological Sciences Research Council (BB/H019774/1). | en_GB |
dc.identifier.citation | Journal of Cell Biology, 2014, Vol. 204, no.3, pp. 343 - 357 | en_GB |
dc.identifier.doi | 10.1083/jcb.201307164 | |
dc.identifier.uri | http://hdl.handle.net/10871/20932 | |
dc.language.iso | en | en_GB |
dc.publisher | Rockefeller University Press | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/24493587 | en_GB |
dc.rights | This is the final version of the article. Available from Rockefeller University Press via the DOI in this record. | en_GB |
dc.subject | Biological Transport, Active | en_GB |
dc.subject | Diffusion | en_GB |
dc.subject | Dyneins | en_GB |
dc.subject | Endosomes | en_GB |
dc.subject | Fungal Proteins | en_GB |
dc.subject | Green Fluorescent Proteins | en_GB |
dc.subject | Hyphae | en_GB |
dc.subject | Kinesin | en_GB |
dc.subject | Microtubules | en_GB |
dc.subject | Models, Biological | en_GB |
dc.subject | Mutation | en_GB |
dc.subject | Polyribosomes | en_GB |
dc.subject | Protein Synthesis Inhibitors | en_GB |
dc.subject | Protein Transport | en_GB |
dc.subject | RNA-Binding Proteins | en_GB |
dc.subject | Stress, Physiological | en_GB |
dc.subject | Ustilago | en_GB |
dc.title | Early endosome motility spatially organizes polysome distribution. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2016-04-04T11:06:04Z | |
dc.identifier.issn | 0021-9525 | |
exeter.place-of-publication | United States | |
dc.description | Published | en_GB |
dc.description | Journal Article | en_GB |
dc.description | Research Support, Non-U.S. Gov't | en_GB |
dc.identifier.journal | Journal of Cell Biology | en_GB |