Show simple item record

dc.contributor.authorHiguchi, Y
dc.contributor.authorAshwin, P
dc.contributor.authorRoger, Y
dc.contributor.authorSteinberg, G
dc.date.accessioned2016-04-04T11:06:04Z
dc.date.issued2014-02-03
dc.description.abstractEarly endosomes (EEs) mediate protein sorting, and their cytoskeleton-dependent motility supports long-distance signaling in neurons. Here, we report an unexpected role of EE motility in distributing the translation machinery in a fungal model system. We visualize ribosomal subunit proteins and show that the large subunits diffused slowly throughout the cytoplasm (Dc,60S = 0.311 µm(2)/s), whereas entire polysomes underwent long-range motility along microtubules. This movement was mediated by "hitchhiking" on kinesin-3 and dynein-driven EEs, where the polysomes appeared to translate EE-associated mRNA into proteins. Modeling indicates that this motor-driven transport is required for even cellular distribution of newly formed ribosomes. Indeed, impaired EE motility in motor mutants, or their inability to bind EEs in mutants lacking the RNA-binding protein Rrm4, reduced ribosome transport and induced ribosome aggregation near the nucleus. As a consequence, cell growth was severely restricted. Collectively, our results indicate that polysomes associate with moving EEs and that "off- and reloading" distributes the protein translation machinery.en_GB
dc.description.sponsorshipThis work was supported by Wellcome Trust (097835/Z/11/Z) and the Biotechnology and Biological Sciences Research Council (BB/H019774/1).en_GB
dc.identifier.citationJournal of Cell Biology, 2014, Vol. 204, no.3, pp. 343 - 357en_GB
dc.identifier.doi10.1083/jcb.201307164
dc.identifier.urihttp://hdl.handle.net/10871/20932
dc.language.isoenen_GB
dc.publisherRockefeller University Pressen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24493587en_GB
dc.rightsThis is the final version of the article. Available from Rockefeller University Press via the DOI in this record.en_GB
dc.subjectBiological Transport, Activeen_GB
dc.subjectDiffusionen_GB
dc.subjectDyneinsen_GB
dc.subjectEndosomesen_GB
dc.subjectFungal Proteinsen_GB
dc.subjectGreen Fluorescent Proteinsen_GB
dc.subjectHyphaeen_GB
dc.subjectKinesinen_GB
dc.subjectMicrotubulesen_GB
dc.subjectModels, Biologicalen_GB
dc.subjectMutationen_GB
dc.subjectPolyribosomesen_GB
dc.subjectProtein Synthesis Inhibitorsen_GB
dc.subjectProtein Transporten_GB
dc.subjectRNA-Binding Proteinsen_GB
dc.subjectStress, Physiologicalen_GB
dc.subjectUstilagoen_GB
dc.titleEarly endosome motility spatially organizes polysome distribution.en_GB
dc.typeArticleen_GB
dc.date.available2016-04-04T11:06:04Z
dc.identifier.issn0021-9525
exeter.place-of-publicationUnited States
dc.descriptionPublisheden_GB
dc.descriptionJournal Articleen_GB
dc.descriptionResearch Support, Non-U.S. Gov'ten_GB
dc.identifier.journalJournal of Cell Biologyen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record