Microscope calibration using laser written fluorescence (article)
Corbett, AD; Shaw, M; Yacoot, A; et al.Jefferson, A; Schermelleh, L; Wilson, T; Booth, M; Salter, PS
Date: 8 August 2018
Article
Journal
Optics Express
Publisher
Optical Society of America
Publisher DOI
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Abstract
There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral ...
There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.
Physics and Astronomy
Faculty of Environment, Science and Economy
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