| dc.contributor.author | Corbett, AD | |
| dc.contributor.author | Shaw, M | |
| dc.contributor.author | Yacoot, A | |
| dc.contributor.author | Jefferson, A | |
| dc.contributor.author | Schermelleh, L | |
| dc.contributor.author | Wilson, T | |
| dc.contributor.author | Booth, M | |
| dc.contributor.author | Salter, PS | |
| dc.date.accessioned | 2018-08-08T13:46:56Z | |
| dc.date.issued | 2018-08-08 | |
| dc.description.abstract | There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes. | en_GB |
| dc.description.sponsorship | Open Innovation Proof of Concept Award (University of Exeter); University Challenge Seed
Fund (University of Oxford) (UCSF 427); Wellcome Trust Strategic Award 107457
(University of Oxford); Engineering and Physical Sciences Research Council
(EP/R004803/01). | en_GB |
| dc.identifier.citation | Vol. 26 (17), pp. 21887-21899. | en_GB |
| dc.identifier.doi | 10.1364/OE.26.021887 | |
| dc.identifier.uri | http://hdl.handle.net/10871/33695 | |
| dc.language.iso | en | en_GB |
| dc.publisher | Optical Society of America | en_GB |
| dc.relation.url | https://doi.org/10.24378/exe.585 | |
| dc.rights | © 2018 Optical Society of America. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/). Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. | |
| dc.subject | Fluorescence microscopy | en_GB |
| dc.subject | Optical standards and testing | en_GB |
| dc.subject | Nanostructure fabrication | en_GB |
| dc.subject | Fluorescent and luminescent materials | en_GB |
| dc.title | Microscope calibration using laser written fluorescence (article) | en_GB |
| dc.type | Article | en_GB |
| dc.identifier.issn | 1094-4087 | |
| dc.description | This is the author accepted manuscript. The final version is available from the Optical Society of America via the DOI in this record. | en_GB |
| dc.description | The dataset associated with this article is located in ORE at: https://doi.org/10.24378/exe.585 | |
| dc.identifier.journal | Optics Express | en_GB |
