NMR Spectral Analysis of Ascorbate-Deficient Mutants of Arabidopsis Thaliana

DSpace/Manakin Repository

Open Research Exeter (ORE)

NMR Spectral Analysis of Ascorbate-Deficient Mutants of Arabidopsis Thaliana

Please use this identifier to cite or link to this item: http://hdl.handle.net/10036/3399


Title: NMR Spectral Analysis of Ascorbate-Deficient Mutants of Arabidopsis Thaliana
Author: Charles Johnson, Felicia
Advisor: Smirnoff, Nicholas
Publisher: University of Exeter
Date Issued: 2011-01-10
URI: http://hdl.handle.net/10036/3399
Abstract: Ascorbate is an important antioxidant, involved in the scavenging of reactive oxygen species (ROS) such as hydrogen peroxide hydrogen. It is also a cofactor for 2-oxoglutarate-dependent dioxygenases, which are involved in the biosynthesis of a number of metabolites. In the current study, Arabidopsis thaliana ascorbate-deficient mutants (vtc1, vtc2, vtc3, and vtc4) were used to investigate the effect of ascorbate deficiency on the metabolic changes. NMR was used as an analytical technique for the metabolite profiling of Arabidopsis thaliana vtc mutants. A total of 45 NMR spectra representing wild type and four mutants of two different growth stages (rosette and flowering) were collected and spectral processed for exploratory analysis. Analysis of variance (ANOVA) and multivariate data analysis (hierarchical clustering) were used to find the differences between different strains and metabolite clustering patterns. Combinations of different parameters were used to find the best method for the analysis of NMR-based metabolites data. The method was developed which allowed making multiple comparison such as two growth stages and five strains. It was found the vtc mutants grouped separately from WT which contained the low ascorbate showing ascorbate deficiency might have some effect on the metabolic level. Two interesting resonances 1.32 and 1.33ppm were separated which were negatively correlated with the ascorbate levels in the vtc mutants. These resonances (1.32 and 1.33) are putatively identified as threonine and lactate. Further investigations are needed to investigate the nature of relationship between ascorbate and threonine and lactate.
Type: Thesis or dissertation
Keywords: Ascorbatevtc mutantsmetabolomicshierarchical clustering analysisANOVA

Please note: Before reusing this item please check the rights under which it has been made available. Some items are restricted to non-commercial use. Please cite the published version where applicable.

Files in this item

Files Size Format View Description
CharlesJohnsonF_fm.pdf 102.4Kb PDF View/Open Front Matter
CharlesJohnsonF.pdf 888.2Kb PDF View/Open Full Text of Dissertation

This item appears in the following Collection(s)


My Account

Local Links