NMR Spectral Analysis of Ascorbate-Deficient Mutants of Arabidopsis Thaliana

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NMR Spectral Analysis of Ascorbate-Deficient Mutants of Arabidopsis Thaliana

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dc.contributor.author Charles Johnson, Felicia en_US
dc.date.accessioned 2012-02-20T11:37:27Z en_US
dc.date.accessioned 2013-03-20T18:46:13Z
dc.date.issued 2011-01-10 en_US
dc.description.abstract Ascorbate is an important antioxidant, involved in the scavenging of reactive oxygen species (ROS) such as hydrogen peroxide hydrogen. It is also a cofactor for 2-oxoglutarate-dependent dioxygenases, which are involved in the biosynthesis of a number of metabolites. In the current study, Arabidopsis thaliana ascorbate-deficient mutants (vtc1, vtc2, vtc3, and vtc4) were used to investigate the effect of ascorbate deficiency on the metabolic changes. NMR was used as an analytical technique for the metabolite profiling of Arabidopsis thaliana vtc mutants. A total of 45 NMR spectra representing wild type and four mutants of two different growth stages (rosette and flowering) were collected and spectral processed for exploratory analysis. Analysis of variance (ANOVA) and multivariate data analysis (hierarchical clustering) were used to find the differences between different strains and metabolite clustering patterns. Combinations of different parameters were used to find the best method for the analysis of NMR-based metabolites data. The method was developed which allowed making multiple comparison such as two growth stages and five strains. It was found the vtc mutants grouped separately from WT which contained the low ascorbate showing ascorbate deficiency might have some effect on the metabolic level. Two interesting resonances 1.32 and 1.33ppm were separated which were negatively correlated with the ascorbate levels in the vtc mutants. These resonances (1.32 and 1.33) are putatively identified as threonine and lactate. Further investigations are needed to investigate the nature of relationship between ascorbate and threonine and lactate. en_GB
dc.identifier.uri http://hdl.handle.net/10036/3399 en_US
dc.language.iso en en_GB
dc.publisher University of Exeter en_GB
dc.rights.embargoreason To allow publication of the research en_GB
dc.subject Ascorbate en_GB
dc.subject vtc mutants en_GB
dc.subject metabolomics en_GB
dc.subject hierarchical clustering analysis en_GB
dc.subject ANOVA en_GB
dc.title NMR Spectral Analysis of Ascorbate-Deficient Mutants of Arabidopsis Thaliana en_GB
dc.type Thesis or dissertation en_GB
dc.date.available 2013-08-20T03:00:11Z
dc.contributor.advisor Smirnoff, Nicholas en_US
dc.publisher.department Biosciences en_GB
dc.type.degreetitle MPhil in Biological Sciences en_GB
dc.type.qualificationlevel Masters Degree en_GB
dc.type.qualificationname MPhil en_GB


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